[Radioimmunoassay of plasma cortisol].
نویسندگان
چکیده
A radioimmunoassay for the determination of plasma cortisol is described. The antiserum against cortisol was produced by the immunization of rabbits with cortisol21-BSA synthesized from cortisol via hemisuccinate derivative. The antiserum obtained had high affinity and high specificity for cortisol. 0.002 ml of plasma diluted with water was used for the assay. After 1,000 dpm of tritiated cortisol was added to compensate for
منابع مشابه
P78: Depression and the Relation of These Symptoms with Plasma Cortisol Level in Individuals Dependent and Independent to Methamphetamine, a Cross-Sectional Study
Methamphetamine is a psychostimulant drug that is highly addictive and causes epigenetic changes that is associated with symptoms of depression. The aim of the present study is comparing age and gender differences in individuals dependent and independent to methamphetamine and examining the relation of depression with the level of cortisol. In a cross-sectional study, 55 methamphetamine users w...
متن کاملParallel radioimmunoassay for plasma cortisol and 11-deoxycortisol.
We describe a direct, rapid, and specific procedure for the parallel radioimmunoassay for cortisol and 11-deoxycortisol in plasma. The plasma sample is used directly, after heat inactivation of the natural cortisol-binding protein. The radioimmunoassay utilizes antibodies generated in rabbits by steroids congugated at their 3-oxo position to thyroglobulin. Ammonium sulfate is used to separate b...
متن کاملAdaptation and validation of a radioimmunoassay kit for measuring plasma cortisol in turbot.
Levels of cortisol in fish blood provide quantitative information on the degree of stress induced by a variety of stressors. It is also useful in describing the social status of individual fish within groups. The commercial production of radioimmunoassay (RIA) kits, such as the DPC Coat-A-Count radioimmunoassay kit, has considerably reduced the effort required for cortisol measurement. These ki...
متن کاملA simple, direct radioimmunoassay for plasma cortisol, featuring a 125I radioligand and a solid-phase separation technique.
A simple, direct radioimmunoassay for cortisol in human serum and plasma is described. An antiserum, raised in sheep to a cortisol-3-(O-carboxymethyl)oxime/bovine serum albumin conjugate, is coupled to microcellulose. No extraction is required because plasma samples and standards are incubated with the antiserum and an 125I radioligand in a low-pH buffer, which denatures cortisol-binding globul...
متن کاملPlasma cortisol in men--relationship with atherosclerosis of retinal arteries.
The production of cortisol increases in acute stress but the effects of chronic stress on plasma cortisol are still controversial. Stress on the other hand plays a role in coronary artery disease (CAD) and carotid atherosclerosis. Since there is no data about plasma cortisol and atherosclerosis of the retinal arteries, the purpose of this study was to explore the relationship between plasma cor...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nihon Naibunpi Gakkai zasshi
دوره 49 10 شماره
صفحات -
تاریخ انتشار 1973